WebFor the treatment of REC8 with λ-PPase, immunoprecipitates obtained with anti-REC8 antibody and protein A ... (10 kDa cutoff; Merck), and the concentration was calculated using the RC-DC Protein Assay kit (Bio-Rad Laboratories) according to the manufacturer’s instructions. Immunocytochemistry of nuclear spreads. Nuclear spreads were ... WebMar 6, 2024 · ATPase Assays. One of the most robust uses of measuring ATPase inhibition is with an ATPase Activity Assay. The protocol developed by BellBrook Labs has been in use for over ten years, with millions of tests performed. All with the goal of first-in-class drugs targeting cancer, autoimmunity pathways, and many other indications.
E. coli Polynucleotide Phosphorylase (PNPase) Assay Kit
WebEach kit provides sufficient reagents for 200 assays. When used as recommended, this kit is ~100-fold more sensitive than either the casein-gel assay or casein A 280 assay. The … WebPPase, a homodimer of a single polypeptide, is a much simpler enzyme that uses energy of the phosphoanhydride bond of pyrophosphate (PP i) to drive proton transport across membranes (9, 11). Both proton pumps are among the most abundant tonoplast proteins (12, 13), indicating that the amount of energy invested into vacuolar transport is ... dragon fruit catering swansea
Lipoprotein Lipase (LPL) Activity Assay - Cell Biolabs
Web*** This assay requires additional sample dilution. Ordering Options Premixed Panels Four premixed panels are available in the new all-in-one kit format.* x-Plex™ Custom Assays (We Mix) Custom premixed assay panels in the new all-in-one kit format are available as 1 x 96 and 10 x 96 kits. Minimum order of two kits applies if ordering a 6-plex ... WebJun 1, 2001 · The method is based on PPase-induced activation of the firefly luciferase activity in the presence of inorganic pyrophosphate (PPi). PPi inhibits the luciferase activity, but in the presence of PPase the luciferase activity is restored and the luminescence output increases. The assay yields linear responses between 8 and 500 mU. WebOct 15, 2024 · PP2A phosphatase activity was determined by using ProFluor ® Ser/Thr PPase Assay Kit (Promega, USA) ... The inhibitory effect of okadaic acid on PP2A activity was also confirmed by a PP2A phosphatase assay kit in Marc-145 cells (Figure 1B). Thus, Marc-145 cells were treated with PP2A inhibitor okadaic acid for 1 h, ... emirates id printed