Phis2自激活

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Webb30 nov. 2024 · 技术原理：2个结构域彼此分离但为功能必需的结构域，单独作用不能激活转录反应，当二者在空间上充分接近时，则呈现完整的GAL4转录因子活性使启动子下游基因（抗性筛选、蓝白斑筛选、营养缺陷型筛选等）得到转录。把需要检测的蛋白质分别与BD和AD融合，形成 bait 融合蛋白（BD- bait）和 prey 融合蛋白（AD-prey），如果 bait … Webb先下结论，不存在可以预测蛋白是否互作的预测方法，小分子和蛋白互作可以通过计算MM-PBSA结合自由能来预测，蛋白-蛋白是否互作需要借助实验手段如IP、等温滴定量热（ITC）或者表面等离子共振（SPR）。. 有了实验基础，可以通过模拟的方式预测蛋白的结 …

pHis2_载体及菌株库_产品中心_奥诺基因-HonorGene-基因研究资 …

WebbpHIS2 PVT4037 2ug Lot no 1901093 Store at -20℃ Expiry date June.2024 pHIS2 Information Promoters: TRP1, T7, T3 Replicon: pUC ori, FI ori Plasmid classification: yeast series, yeast one hybrid vector Plasmid size: 7207bp … Webb中国微生物菌种查询网专业提供pHIS2查询与购买，编号为bio-109465,微生物菌种查询网(The Website of Microorganism for Search)是集菌种信息、细胞信息、质粒信息及培养基信息于一体的微生物相关查询网站,并提供美国ATCC、日本JCM等进口品牌的菌种购买服务,数据庞大，内容丰富，欢迎您的查阅！ small holiday trailers for sale

Transcription Factor-Centered Yeast One-Hybrid Assay

Webb8 juli 2024 · pHis2 编号载体名称北京华越洋生物VECT2110 pHis2 pHis2 载体基本信息出品公司: Clontech 载体名称: pHis2 质粒类型: 酵母单杂交载体高拷贝/低拷贝: -- 启动子: -- 克隆方法: 多克隆位点，限制性内切酶载体大小: 7207bp 5' 测序引物及序列: -- 3' 测序引物及序列: -- 载体标签 ... http://www.qualityard.com/upload/2024-12-17/dbd8dacfb1f8d0626e1c10bc4840ba05.pdf http://www.bioon.com.cn/doc/showarticle.asp?newsid=101857 small holidays in 2023

pGADT7-T Vector酵母双杂交对照载体 - 上海懋康生物科技有限公司

Webbactivation domain in pGADT7-Rec2. The target DNA sequence, or Bait Sequence, is cloned into pHIS2.1 as one copy or tandem repeats. Interaction between a DNA-binding protein … Webb1 juni 2024 · Clone the products of the PCR reactions carried out in step 3 into the pHIS2 T-vector obtained in step 5. The T-A ligation system includes 0.2 μg of T-vector of pHIS2, 0.5 μL of PCR products (Y1 + Y3 and Y2 + Y3), 1 μL of 10× ligation buffer, 3 U of T4 ligase, and PEG6000 at final concentration of 10% (w/v). sonic boom is back sonicWebb1 mars 2024 · MdGSTF6-p-pHIS2 vectors were first grown on −Trp/−His medium containing 3-amino-1,2,4-triazole (3-AT) for screening. The concentration of 3-AT used was 100 mM. sonic boom in sonic 1 2013

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Phis2自激活

Webb载体描述. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One- Hybrid Library Construction & Screening Kit. It contains a HIS3 nutritional reporter gene, located downstream of a multiple cloning ... WebbpHIS2 PVT4037 2ug Lot no 1901093 Store at -20℃ Expiry date June.2024 pHIS2 Information Promoters: TRP1, T7, T3 Replicon: pUC ori, FI ori Plasmid classification: …

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http://www.honorgene.com/product_list/carrier_library/123112.html Webb19 nov. 2012 · 是选取atg上游2000的片段，然后整个连到phis2载体上吗？我之前做了直接取ATG上游1.5k构建到phis2上，感觉不合适。最好还是找到自己转录因子的结合motif …

http://muchong.com/t-9775583-1 WebbpHis2载体价格1000元，pHis2载体质粒图谱(Vector map),载体序列(Sequence)见下文，载体质粒抗性为Kanamycin，质粒大小为7207bp。pHis2是酵母单杂交载体的pHis2。

Webb泰斯拓生物向社会各界提供古菌、细菌、放线菌、酵母菌、丝状真菌等共享菌株资源，. 包括模式菌株、标准菌株（质控菌株）、生产菌株、以及用于研发的菌株，. 覆盖了科研、教育、农林、工业、海洋等行业的科研、生产和检验用菌株。. 且我司提供国外各 ... Webb11 juli 2014 · 常用载体及通用测序引物,测序通用引物,华大测序通用引物,载体通用引物,petduet 1测序引物,通用引物,测序引物,pet28a测序引物,m13通用引物,细菌通用引物,病毒通用引物. VectorPrimer (F)Primer (R)抗性载体大小 (bp)EZ-TM13-47RV-MAmp+2984LZRS-RfALZRS-FLZRS-R11100p3xFLAGMV14CMV30 (834bp)CMV24 ...

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Webb23 apr. 2024 · 答：标准设置为30℃孵育30 min，然后42℃热激20 min。孵育时间在3 h以内，热激不超过30 min都可以得到不错的转化效果。 Coolaber的Super转化Kit经过优化及检测证明，将孵育热激合并，条件设定为30℃，45-60 min同样能达到理想的转化效果。问6：转化产物用无菌水和生理盐水重悬有什么区别？答：重悬后直接涂板，生理盐水和无菌水 … sonic boom king of bee botsWebb利用酵母反式单杂交、酵母单杂交、烟草瞬时转化和染色体免疫共沉淀技术分析ThWRKY4蛋白对ARR1AT元件的结合特性及对基因的表达调控情况。. 结果表明,ThWRKY4能够结合一个新的顺式作用元件ARR1AT（AATCG）;ThWRKY4能够特异性结合ARR1AT元件,而不能结合ARR1AT的突变元件AM1 ... sonic boom kentWebb5 nov. 2015 · 酵母双杂交 AH109,Y187. 下面所有质粒和菌株，均为本公司实验室亲自验证过，可以提供，载体来源于 promega,addgene,clontech,novagen,invitrogen 可提供质粒图谱，可提供近万种质粒载体、菌株、基因和细胞株, 并可提供实验技术外包服务, 如基因克隆、质粒构建、蛋白原核 ... sonic boom knuck knuck who\u0027s hereWebb质粒简介：. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One-Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene,located downstream of a multiple ... small hollieshttp://www.coolaber.com/Column.asp?Model=Content_Detail&Column_ID=29596&C_ID=284549825 small holiday parks in cornwallWebbpHIS2 can be maintained in both yeast and bacteria. It contains an autonomous replication se-quence ( ARS4) and TRP1 nutritional marker for replication and selection in yeast (1, … small holiday stickersWebb24 jan. 2024 · Jan 14, 2015. Answer. Yeast one-hybrid traditionally only measures DNA binding, not activation or repression (TFs always activate transcription when they bind to target DNA in Y1H because they are ... small holley 4 barrel carb