Deuterium raman imaging for lipid analysis
WebD38-cholesterol as a Raman active probe for imaging intracellular cholesterol storage Alba Alfonso-García, a, Simon G. Pfisterer, b, Howard Riezman, c Elina Ikonen, b and Eric O. Potma d, * a University of California, Irvine, Department of Biomedical Engineering, Irvine, California 92697, United States b University of Helsinki, Finland and Minerva … WebHere we report a microscopy technique for the optical imaging, via the spectral tracing of deuterium (STRIDE), of diverse macromolecules derived from glucose. Based on stimulated Raman-scattering imaging, STRIDE visualizes the metabolic dynamics of newly synthesized macromolecules, such as DNA, protein, lipids and glycogen, via the …
Deuterium raman imaging for lipid analysis
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WebOct 8, 2024 · Here, we present a novel Raman microscopy-based approach for quantifying the physical properties of intracellular lipids under deuterium-labeled fatty acid … WebAbstract. We generated a highly deuterated cholesterol analog (D38-cholesterol) and demonstrated its use for selective vibrational imaging of cholesterol storage in mammalian cells. D38-cholesterol produces detectable signals in stimulated Raman scattering (SRS) imaging, is rapidly taken up by cells, and is efficiently metabolized by acyl-CoA ...
WebOct 1, 2024 · Therefore, deuterium has been used as a Raman tag for in vivo Raman imaging of lipids composed of many C–H bonds. In particular, among the deuterium … WebDec 27, 2024 · Quantitative methods to precisely measure cellular states in vivo have become increasingly important and desirable in modern biology. Recently, stimulated Raman scattering (SRS) microscopy has emerged as a powerful tool to visualize small biological molecules tagged with alkyne (C≡C) or carbon–deuterium (C–D) bonds in the …
WebApr 29, 2024 · To further ensure that the Raman spectrum of each macromolecule discovered above is reproducible and accurate, we obtained in situ lipid and protein … WebJul 28, 2024 · Raman microscopy is a powerful imaging tool for observing endogenous biomolecules, as it can detect molecular vibrations in cells without exogenous labeling. 1 …
WebDec 10, 2024 · Raman–DIP analysis at 24 h reveals that the overall active ratios in volunteer 1 and 2 were 76 ± 1.0% and 30 ± 2.0% respectively ... cm −1) at the C–D region, lipids (2874 cm −1), proteins (2928 cm −1) and DNA (2962 cm −1) at the C–H region and deuterium shifted bands at saturated lipids (1080 cm −1), carbohydrates (1097 ...
WebApr 29, 2024 · To further ensure that the Raman spectrum of each macromolecule discovered above is reproducible and accurate, we obtained in situ lipid and protein Raman spectra from [D 7]-glucose-labelled cells ... flowey es asrielWebOct 1, 2016 · In Raman analysis, the red-shift caused by 15 N substitution is not as large as that caused by 13 C-replacement (Table 1) [21], and most red-shifts of the Raman bands are related to nucleic acids [21, 24, 36] (Figure 2b). ... Deuterium Raman imaging for lipid analysis. 2024, Current Opinion in Chemical Biology ... flowey evil smileWebMay 20, 2024 · Deuterium Raman imaging for lipid analysis. Article. Full-text available. Oct 2024; CURR OPIN CHEM BIOL; Syusuke Egoshi; Kosuke Dodo; Mikiko Sodeoka; Raman microscopy has been used to deduce ... flowey evilWebOct 1, 2024 · Deuterium Raman imaging for lipid analysis Egoshi et al. 3 www.sciencedirect.com Current Opinion in Chemical Biology 2024, 70: 102181 number … floweye安全域流监控系统WebApr 10, 2024 · The Raman shift was first set to 2,870 cm −1, to target the CH 2 stretching vibrational mode, imaging the skin's microscale lipid structure. Next, the Raman shift was automatically toggled to 2,250 cm −1 to target the nitrile stretch of Rux. Imaging was performed using a 20 × 0.8 NA microscope objective (UPLXAPO20X, Olympus, Tokyo, … green cab beckley wvWebEnter the email address you signed up with and we'll email you a reset link. floweye命令WebMar 30, 2015 · We also performed Raman imaging of lipid membrane ... Osaka University) for his support for the analysis of Raman imaging data. We thank Dr. Taisuke Ota (Nanophoton) for technical support for the … green cabinets black counter